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rabbit anti vgat  (Proteintech)


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    Structured Review

    Proteintech rabbit anti vgat
    Rabbit Anti Vgat, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+vgat/VGAT+Antibody/pm41806666-100-26-30
    Average 93 stars, based on 26 article reviews
    rabbit anti vgat - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: Microglial activation drives neuronal dysregulation in alcohol-induced seizure susceptibility.
    Article Snippet: .. After being blocked in PBS containing 5% normal donkey serum (NDS; Jackson ImmunoResearch 017-000- 121), 1% bovine serum albumin (BSA; Sigma-Aldrich, USA), and 0.3% Triton X-100 (Sigma-Aldrich, USA) for 2 h at room temperature, brain sections were incubated with primary antibodies: rabbit anti-Iba1 (Proteintech, 10,904-1-AP, USA), rabbit anti-GFAP (Proteintech, 16,825-1-AP, USA), mouse anti-GAD67 (Proteintech, 67,648-1-Ig, USA), mouse anti-NeuN (Proteintech, 66,836-1-Ig, USA), rabbit anti-VGAT (Proteintech, 14,471- 1-AP, USA), rabbit anti-VGLUT1 (Proteintech, 55,491-1-AP, USA), mouse anti-GFAP (Proteintech, 60,190–1- Ig, USA) rabbit anti-C3 (Proteintech, 21,337-1-AP, USA) and rabbit anti-CaMKII (Proteintech, 13,730-1-AP, USA) overnight at 4 °C in blocking solution. .. Brain slices were washed in PBS (3 × 10 min) and incubated with corresponding secondary antibodies [Alexa Fluor 546 anti-rabbit IgG (Thermo Fisher Scientific, A10040, USA), Alexa Fluor 488 anti-mouse IgG (Thermo Fisher Scientific, A21202, USA)] for 1 h at room temperature.

    Article Title: Cognitive behavioral dysregulation induced by low-level lead, cadmium, and mercury co-exposure is linked to AMPA receptor-associated with E/I imbalance.
    Article Snippet: .. With non-specific antibody binding sites blocking, sections were incubated with the primary antibody: mouse anti-c-Fos (1:1000, ab208942, Abcam), rabbit anti-vGluT1 (1:200, DF13657, Affinity Biosciences Ltd.), and/or rabbit anti-vGAT (1:500, 14471–1-AP, Proteintech) overnight at 4 ◦C. .. For immunohistochemistry, following the incubation of Peroxidase AffiniPure Goat Anti-Mouse IgG (H+L) (1:200, 115–035–003, Jackson ImmunoResearch), the sections were stained with DAB (ALI-9017, ZSGB-Bio, China).

    Article Title: Microglial activation drives neuronal dysregulation in alcohol-induced seizure susceptibility
    Article Snippet: .. After being blocked in PBS containing 5% normal donkey serum (NDS; Jackson ImmunoResearch 017-000-121), 1% bovine serum albumin (BSA; Sigma-Aldrich, USA), and 0.3% Triton X-100 (Sigma-Aldrich, USA) for 2 h at room temperature, brain sections were incubated with primary antibodies: rabbit anti-Iba1 (Proteintech, 10,904-1-AP, USA), rabbit anti-GFAP (Proteintech, 16,825-1-AP, USA), mouse anti-GAD67 (Proteintech, 67,648-1-Ig, USA), mouse anti-NeuN (Proteintech, 66,836-1-Ig, USA), rabbit anti-VGAT (Proteintech, 14,471-1-AP, USA), rabbit anti-VGLUT1 (Proteintech, 55,491-1-AP, USA), mouse anti-GFAP (Proteintech, 60,190–1-Ig, USA) rabbit anti-C3 (Proteintech, 21,337-1-AP, USA) and rabbit anti-CaMKII (Proteintech, 13,730-1-AP, USA) overnight at 4 °C in blocking solution. .. Brain slices were washed in PBS (3 × 10 min) and incubated with corresponding secondary antibodies [Alexa Fluor 546 anti-rabbit IgG (Thermo Fisher Scientific, A10040, USA), Alexa Fluor 488 anti-mouse IgG (Thermo Fisher Scientific, A21202, USA)] for 1 h at room temperature.

    Article Title: Exercise Ameliorates Fluoride-induced Anxiety- and Depression-like Behavior in Mice: Role of GABA.
    Article Snippet: Fluoride exposure caused anxietyand depression-like behavior in mice.. Meanwhile, exercise contributes to relieve anxiety and depression.. However, the effects of exercise on anxietyand depression-like behavior in fluorosis mice remain unclear.

    Blocking Assay:

    Article Title: Microglial activation drives neuronal dysregulation in alcohol-induced seizure susceptibility.
    Article Snippet: .. After being blocked in PBS containing 5% normal donkey serum (NDS; Jackson ImmunoResearch 017-000- 121), 1% bovine serum albumin (BSA; Sigma-Aldrich, USA), and 0.3% Triton X-100 (Sigma-Aldrich, USA) for 2 h at room temperature, brain sections were incubated with primary antibodies: rabbit anti-Iba1 (Proteintech, 10,904-1-AP, USA), rabbit anti-GFAP (Proteintech, 16,825-1-AP, USA), mouse anti-GAD67 (Proteintech, 67,648-1-Ig, USA), mouse anti-NeuN (Proteintech, 66,836-1-Ig, USA), rabbit anti-VGAT (Proteintech, 14,471- 1-AP, USA), rabbit anti-VGLUT1 (Proteintech, 55,491-1-AP, USA), mouse anti-GFAP (Proteintech, 60,190–1- Ig, USA) rabbit anti-C3 (Proteintech, 21,337-1-AP, USA) and rabbit anti-CaMKII (Proteintech, 13,730-1-AP, USA) overnight at 4 °C in blocking solution. .. Brain slices were washed in PBS (3 × 10 min) and incubated with corresponding secondary antibodies [Alexa Fluor 546 anti-rabbit IgG (Thermo Fisher Scientific, A10040, USA), Alexa Fluor 488 anti-mouse IgG (Thermo Fisher Scientific, A21202, USA)] for 1 h at room temperature.

    Article Title: Cognitive behavioral dysregulation induced by low-level lead, cadmium, and mercury co-exposure is linked to AMPA receptor-associated with E/I imbalance.
    Article Snippet: .. With non-specific antibody binding sites blocking, sections were incubated with the primary antibody: mouse anti-c-Fos (1:1000, ab208942, Abcam), rabbit anti-vGluT1 (1:200, DF13657, Affinity Biosciences Ltd.), and/or rabbit anti-vGAT (1:500, 14471–1-AP, Proteintech) overnight at 4 ◦C. .. For immunohistochemistry, following the incubation of Peroxidase AffiniPure Goat Anti-Mouse IgG (H+L) (1:200, 115–035–003, Jackson ImmunoResearch), the sections were stained with DAB (ALI-9017, ZSGB-Bio, China).

    Article Title: Microglial activation drives neuronal dysregulation in alcohol-induced seizure susceptibility
    Article Snippet: .. After being blocked in PBS containing 5% normal donkey serum (NDS; Jackson ImmunoResearch 017-000-121), 1% bovine serum albumin (BSA; Sigma-Aldrich, USA), and 0.3% Triton X-100 (Sigma-Aldrich, USA) for 2 h at room temperature, brain sections were incubated with primary antibodies: rabbit anti-Iba1 (Proteintech, 10,904-1-AP, USA), rabbit anti-GFAP (Proteintech, 16,825-1-AP, USA), mouse anti-GAD67 (Proteintech, 67,648-1-Ig, USA), mouse anti-NeuN (Proteintech, 66,836-1-Ig, USA), rabbit anti-VGAT (Proteintech, 14,471-1-AP, USA), rabbit anti-VGLUT1 (Proteintech, 55,491-1-AP, USA), mouse anti-GFAP (Proteintech, 60,190–1-Ig, USA) rabbit anti-C3 (Proteintech, 21,337-1-AP, USA) and rabbit anti-CaMKII (Proteintech, 13,730-1-AP, USA) overnight at 4 °C in blocking solution. .. Brain slices were washed in PBS (3 × 10 min) and incubated with corresponding secondary antibodies [Alexa Fluor 546 anti-rabbit IgG (Thermo Fisher Scientific, A10040, USA), Alexa Fluor 488 anti-mouse IgG (Thermo Fisher Scientific, A21202, USA)] for 1 h at room temperature.

    Binding Assay:

    Article Title: Cognitive behavioral dysregulation induced by low-level lead, cadmium, and mercury co-exposure is linked to AMPA receptor-associated with E/I imbalance.
    Article Snippet: .. With non-specific antibody binding sites blocking, sections were incubated with the primary antibody: mouse anti-c-Fos (1:1000, ab208942, Abcam), rabbit anti-vGluT1 (1:200, DF13657, Affinity Biosciences Ltd.), and/or rabbit anti-vGAT (1:500, 14471–1-AP, Proteintech) overnight at 4 ◦C. .. For immunohistochemistry, following the incubation of Peroxidase AffiniPure Goat Anti-Mouse IgG (H+L) (1:200, 115–035–003, Jackson ImmunoResearch), the sections were stained with DAB (ALI-9017, ZSGB-Bio, China).

    other:

    Article Title: TMEM251 loss-induced autophagy dysfunction in the anterior cingulate cortex contributes to chronic postoperative pain
    Article Snippet: Rabbit anti-VGAT , Proteintech Biotechnology , Cat# 14471-1-AP.



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